Research Profile

John LaCava, Ph.D.

Group Leader, Associate Professor, European Research Institute for the Biology of Ageing
Research Associate Professor, The Rockefeller University
Managing Director, B13LOGY LLC

Emerging Lines of Investigation

Major Funding (previous 5 years)


Dou, Y., S. Kalmykova, M. Pashkova, M. Oghbaie, H. Jiang, K.R. Molloy, B.T. Chait, M.P. Rout, D. Fenyö, T.H. Jensen, I. Altukhov, & J. LaCava. 2020. Affinity proteomic dissection of the human nuclear cap-binding complex interactome. Nucleic Acids Research. DOI: 10.1093/nar/gkaa743

Dou, Y., I. Barbosa, H. Jiang, C. Iasillo, K.R. Molloy, W. Schulze, S. Cusack, M. Schmid, H. Le Hir, J. LaCava, & T.H. Jensen. 2020. NCBP3 positively impacts mRNA biogenesis. Nucleic Acids Research. DOI: 10.1093/nar/gkaa744

Kagan, J., R.L. Moritz, R. Mazurchuk, J.H. Lee, P.V. Kharchenko, O. Rozenblatt-Rosen, E. Ruppin, F. Edfors, F. Ginty, Y. Goltsev, J.A. Wells, J. LaCava, J.L. Riesterer, R.N. Germain, T. Shi, M.S. Chee, B.A. Budnik, J.R. Yates III, B.T. Chait, J.R. Moffitt, R.D. Smith, & S. Srivastava. 2020. National Cancer Institute Think-Tank Meeting Report on Proteomic Cartography and Biomarkers at the Single-Cell Level: Interrogation of Premalignant Lesions. Journal of Proteome Research. DOI: 10.1021/acs.jproteome.0c00021

Ardeljan, D., X. Wang, M. Oghbaie, M.S. Taylor, D. Husband, V. Deshpande, J.P. Steranka, M. Gorbounov, W.R. Yang, B. Sie, H.B. Larman, H. Jiang, K.R. Molloy, I. Altukhov, Z. Li, W. McKerrow, D. Fenyö, K.H. Burns, & J. LaCava. 2020. LINE-1 ORF2p expression is nearly imperceptible in human cancers. Mobile DNA. DOI: 10.1186/s13100-019-0191-2


Mustelin, T., K. Ukadike, J. LaCava, & M. S. Taylor. 2019. To the editor in reply to Dr. Crow’s letter. Arthritis Rheumatology. DOI: 10.1002/art.41104

Carter, V., J. LaCava, M.S. Taylor, S.Y. Liang, C. Mustelin, K.C. Ukadike, A. Bengtsson, C. Lood, & T. Mustelin. 2019. High prevalence and disease correlation of autoantibodies against p40 encoded by long interspersed nuclear elements (LINE-1) in systemic lupus erythematosus. Arthritis & Rheumatology. DOI: 10.1002/art.41054

Jiang, H., M. S. Taylor, K. R. Molloy, I. Altukhov, & J. LaCava. 2019. Identification of RNase-sensitive LINE-1 Ribonucleoprotein Interactions by Differential Affinity Immobilization. Bio-protocol. DOI: 10.21769/BioProtoc.3200

LaCava, J. 2019. RNA Binding Proteins as Regulators of Retrotransposon‐Induced Exonization. Bioessays. DOI: 10.1002/bies.201800263


Pronier, E., P. Cifani, T.R. Merlinsky, K.B. Berman, A.V.H. Somasundara, R.K. Rampal, J. LaCava, K. Wei, F. Pastore, J. Maag, J. Park, R. Koche, A. Kentsis, & R.L. Levine. 2018. Targeting the CALR Interactome in Myeloproliferative Neoplasms. JCI Insight. DOI 10.1172/jci.insight.122703

Chan, H.L., F. Beckedorff, J. Garcia-Huidobro, Y. Zhang, H. Jiang, A. Colaprico, M.E. Figueroa, J. LaCava, R. Shiekhattar, & L. Morey. 2018. Polycomb Complexes Associate with Enhancers and Promote Oncogenic Transcriptional Programs in Cancer through Multiple Mechanisms. Nature Communications. DOI: 10.1038/s41467-018-05728-x

Zinoviev, A., A. Goyal, S. Jindal, J. LaCava, A.A. Komar, M.V. Rodnina, C.U.T. Hellen, & T.V. Pestova. 2018. Functions of unconventional mammalian translational GTPases GTPBP1 and GTPBP2. Genes & Development. DOI: 10.1101/gad.314724.118

Taylor, M.S., I. Altukhov, K.R. Molloy, P. Mita, H. Jiang, E.M. Adney, A. Wudzinska, S. Badri, D. Ischenko, G. Eng, K.H. Burns, D. Fenyö, B.T. Chait, D. Alexeev, M.P. Rout, J.D. Boeke, & J. LaCava. 2018. Dissection of affinity captured LINE-1 macromolecular complexes. eLife. DOI: 10.7554/eLife.30094

Mita, P., A. Wudzinska, X. Sun, J. Andrade, S. Nayak, D.J. Kahler, S. Badri, J. LaCava, B. Ueberheide, C.Y. Yun, D. Fenyö, & J.D Boeke. 2018. LINE-1 protein localization and functional dynamics during the cell cycle. eLife. DOI: 10.7554/eLife.30058

Winczura, K., M. Schmid, C. Iasillo, K.R. Molloy, L.M. Harder, J.S. Andersen, J. LaCava, & T.H. Jensen. 2018. Characterizing ZC3H18, a multi-domain protein at the interface of RNA production and destruction decisions. Cell Reports. DOI: 10.1016/j.celrep.2017.12.037


Ma, Y., L. Silveri, J. LaCava, & S. Dokudovskaya. 2017. Tumor suppressor NPRL2 induces ROS production and DNA damage response. Scientific Reports. DOI: 10.1038/s41598-017-15497-0

Santanach, A., E. Blanco, H. Jiang, K.R. Molloy, M. Sansó, J. LaCava, L. Morey, & L. Di Croce. 2017. The Polycomb group protein CBX6 is an essential regulator of embryonic stem cell identity. Nature Communications. DOI: 10.1038/s41467-017-01464-w

Domanski, M., & J. LaCava. 2017. RNA Degradation Assay Using RNA Exosome Complexes, Affinity-purified from HEK-293 Cells. Bio-protocol. DOI: 10.21769/BioProtoc.2239

Domanski, M., & J. LaCava. 2017. Affinity Purification of the RNA Degradation Complex, the Exosome, from HEK-293 Cells. Bio-protocol. DOI: 10.21769/BioProtoc.2238


Domanski, M., P. Upla, W.J. Rice, K.R. Molloy, N.E. Ketaren, D.L. Stokes, T.H. Jensen, M.P. Rout, & J. LaCava. 2016. Purification and Analysis of Endogenous Human RNA Exosome Complexes. RNA. DOI: 10.1261/rna.057760.116

LaCava, J., H. Jiang, & M.P. Rout. 2016. Protein complex affinity capture from cryomilled mammalian cells. Journal of Visualized Experiments. DOI: 10.3791/54518


Hakhverdyan, Z., M. Domanski, L. Hough, A.A. Oroskar, A.R. Oroskar, D.J. Dilworth, K.R. Molloy, V. Sherman, J.D. Aitchison, B.T. Chait, T.H. Jensen, M.P. Rout, & J. LaCava. 2015. Rapid, Optimized Interactomic Screening. Nature Methods. DOI: 10.1038/nmeth.3395

LaCava, J., K. R. Molloy, M. S. Taylor, M. Domanski, B. T. Chait, & M. P. Rout. 2015. Affinity proteomics to study endogenous protein complexes: Pointers, pitfalls, preferences and perspectives. BioTechniques. DOI: 10.2144/000114262


Dai, L., J. LaCava, M.S. Taylor, & J.D. Boeke. 2014. Expression and detection of LINE-1 ORF-encoded proteins. Mobile Genetic Elements. DOI: 10.4161/mge.29319


Andersen, P.R., M. Domanski, M.S. Kristiansen, H. Storvall, E. Ntini, C. Verheggen, J. Bunkenborg, I. Poser, M. Hallais, R. Sandberg, A. Hyman, J. LaCava, M.P. Rout, J.S. Andersen, E. Bertrand, & T.H. Jensen. 2013. The human cap-binding complex is functionally connected to the nuclear RNA exosome. Nature Structural & Molecular Biology. DOI: 10.1038/nsmb.2703

Taylor, M.S., J. LaCava, P. Mita, K.R. Molloy, C.R.L. Huang, D. Li, E.M. Adney, H. Jiang, K.H. Burns, B.T. Chait, M.P. Rout, J.D. Boeke, & L. Dai. 2013. Affinity proteomics reveals human host factors implicated in discrete stages of LINE-1 retrotransposition. Cell. DOI: 10.1016/j.cell.2013.10.021

LaCava, J., N. Chandramouli, H. Jiang, & M.P. Rout. 2013. Improved native isolation of endogenous Protein A-tagged protein complexes. BioTechniques. DOI: 10.2144/000114012


Domanski, M., K. Molloy, H. Jiang, B.T. Chait, M.P. Rout, T.H. Jensen, & J. LaCava. 2012. Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels. BioTechniques. DOI: 10.2144/000113864


Houseley, J., J. LaCava, & D. Tollervey. 2006. RNA-quality control by the exosome. Nature Reviews Molecular Cell Biology. DOI: 10.1038/nrm1964


LaCava, J., J. Houseley, C. Saveanu, E. Petfalski, E. Thompson, A. Jacquier, & D. Tollervey. 2005. RNA degradation by the exosome is promoted by a nuclear polyadenylation complex. Cell. DOI: 10.1016/j.cell.2005.04.029


The Eukaryotic Exosome: Methods and Protocols. 2020. Methods in Molecular Biology Series (Springer). Vol: 2062. Eds. LaCava, J & Vaňáčová, Š. DOI: 10.1007/978-1-4939-9822-7

Winczura, K., M. Domanski & J. LaCava. 2020. Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes. In J. LaCava & Š. Vaňáčová (Eds.), The Eukaryotic RNA Exosome: Methods and Protocols. Springer. DOI: 10.1007/978-1-4939-9822-7_15


LaCava, J., J. Fernandez-Martinez, Z. Hakhverdyan, & M.P. Rout. 2016. Protein Complex Purification by Affinity Capture. In B. Andrews, C. Boone, T. Davis, & S. Fields (Eds.), Budding Yeast: A Laboratory Manual. Cold Spring Harbor Laboratory Press. DOI: 10.1101/pdb.top077545. Includes 3 companion protocols: DOI: 10.1101/pdb.prot087932; DOI: 10.1101/pdb.prot087940; DOI: 10.1101/pdb.prot087957

Taylor, M.S., J. LaCava, L. Dai, P. Mita, K.H. Burns, M.P. Rout, & J.D. Boeke. Characterization of L1-RNPs. 2016. In J.L. Garcia (Ed.), Transposons and Retrotransposons: Methods and Protocols. Springer. DOI: 10.1007/978-1-4939-3372-3_20


Oroskar, A.A., A.R. Oroskar, J.P. LaCava, & M.P. Rout . 2015. Apparatus and Method for Parallel Collection and Analysis of the Proteome and Complex Compositions. US Patent No. 9,084,994.