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Protocols
Interactome Screening Protocols
2015
Rapid, optimized interactomic screening – original method and results [supplement, protocols]
Affinity proteomics to study endogenous protein complexes – practical guide
General Mammalian Protocols
2016
Protein Complex Affinity Capture from Cryomilled Mammalian Cells – original method and results [supplement]
2012
Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels – original method and results [supplement]
RNA exosome protocols
2020
Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes
2017
RNA Degradation Assay Using RNA Exosome Complexes, Affinity-purified from HEK-293 Cells
Affinity Purification of the RNA Degradation Complex, the Exosome, from HEK-293 Cells
2016
Purification and analysis of endogenous human RNA exosome complexes – original method and results
General Yeast Protocols
2016
Protein Complex Purification by Affinity Capture – topic introduction
- Optimized Affinity Capture of Yeast Protein Complexes – protocol
- Native Elution of Yeast Protein Complexes Obtained by Affinity Capture – protocol
- Density Gradient Ultracentrifugation to Isolate Endogenous Protein Complexes after Affinity Capture – protocol
2013
Improved Native Isolation of Endogenous Protein A-Tagged Protein Complexes – original method and results [supplement, protocol]